Fluorescent Cell Counting

Here is our protocol for cell counting with a twist.

Fluorescent cell counting. Wash your cells with pbs. Fluorescent cell counting kit fcck is utilized for the fluorometric detection of living cell numbers. If you have a cell counter with fluorescent capabilities like we do there is a way around it without adding additional steps to the protocol. Flow cytometric cell counting utilizes a sample with a known concentration of fluorescent beads.

Bright field green fluorescent and red fluorescent channels were used. Cell counting using a fluorescent dye. Add an appropriate amount of trypsin. From the results the total fluorescence per cell is then analyzed using excel sheet.

Because the concentration of beads is known the volume of sample analyzed can be easily computed. Biotek s imaging and microscopy products can count cells using a number of microscopy methods including fluorescence microscopy using fluorometric probes that intercalate with nuclear dna color brightfield for trypan blue based viability measurements and label free cell counting using either phase contrast or high contrast brightfield. The sample is run through a flow cytometer and set to stop after a predetermined number of beads are analyzed.